@article{Bagali_Kakhandaki_Karigoudar_Gajul_Mantur_Shahapur_2020, title={Detection of Metallo beta - lactamases among Enterobacteriaceae isolates at a tertiary care hospital, south India}, volume={4}, url={https://littlebaypublishers.com/index.php/seajournalms/article/view/65}, DOI={10.5281/zenodo.4019977}, abstractNote={<p><strong>Background</strong><strong>:</strong></p> <p>The spread of carbapenem resistant bacteria has caused grave concern due to the limited choice in antibiotics for treating infections caused by them. The emergence of  metallo beta-lactamse (MBL) producing  gram negative bacilli poses a therapeutic challenge and is of serious concern for infection control in hospital environment.</p> <p><strong>Materials & Methods:</strong></p> <p>A total of 475 non repeat clinical isolates of family <em>Enterobacteriaceae </em>were included in the study. Resistance to imipenem was determined in isolates by disc diffusion & minimum inhibitory concentration (MIC) method. Imipenem resistant  isolates  were tested for MBL production by combined disc diffusion test and modified Hodge test.</p> <p><strong>Results:</strong></p> <p>Out of the 475 <em>Enterobacteriaceae</em> strains,  20 showed resistance to imipenem. MBL activity was detected in all 20 (4.2%) isolates by combined disc diffusion test, in 18 isolates by modified Hodge test. The MBL producing isolates included clinical strains of <em>Klebsiella spp</em> (45%), <em>E. coli</em> (40%), <em>Citrobacter spp</em> (15%).  Majority of the MBL isolates were from Intensive care unit (65%), from patients with comorbid conditions and with invasive devices.  MBL producing isolates showed high level of resistance to aminoglycosides and fluoroquinolones but all were susceptible to colistin.</p> <p><strong>Conclusion</strong>:</p> <p>The need of the hour is to detect MBL producing isolates for better patient outcomes, to execute prompt infection control measures and decrease the escalation of resistance.</p> <p><em><strong>Keywords</strong>:  Imipenem resistance, Phenotypic methods, Colistin sensitivity</em></p>}, number={3}, journal={South East Asia Journal of Medical Sciences}, author={Bagali, Smitha and Kakhandaki, Laxmi and Karigoudar, Rashmi and Gajul, Shivali V and Mantur, Prakash G and Shahapur, Praveen R}, year={2020}, month={Jul.}, pages={1–5} }