Comparison of the Toxogen® rapid test with ELISA immunoenzymatic technique for toxoplasmosis serology in pregnant women

Author(s)
Francine Isabelle Rakotonindrina(1*), Rakotomalala Rivo S(2), Tsatoromila Fenosoa A(3), Rabenandrianina Tahirimalala(4), Rajaonatahina Davidra(5), Rakotovao Luc A(6), Rakoto Alson Olivat A(7), Rasamindrakotroka Andry(8), Randriamanantany Zely(9), Razanakolona Lala R S(10)
(*) Corresponding Author

Abstract

The aim of this study was to compare the technical performance of the Toxogen® rapid test against the ELISA technique for serological screening and/or serological monitoring of toxoplasmosis in pregnant women.

Methods and material: A prospective, descriptive and analytical study was conducted over a period of 7 months from January to July 2018. One hundred and eighty-two toxoplasmosis serologies were carried out on 144 pregnant women followed in two hospitals in Mahajanga and in a private laboratory in Antananarivo. Two serologies were performed for each case, the first by the rapid test Toxogen®, a latex agglutination test, and the second by the IgG-ELISA and IgM-ELISA technique.

Results: The Toxogen® rapid test had a sensitivity of 62.5%, a specificity of 97.1%, a Positive Predictive Value of 94.3%, a Negative Predictive Value of 76.7% compared to the IgG-ELISA technique. The correlation between the dilution titer by the Toxogen® rapid test and the optical density (OD) of anti-toxoplasmics IgG by the ELISA technique was very low (r² = 0.17, p <0.001). The crude prevalence of toxoplasmosis was 30,6% (n=44) by the Toxogen® rapid test and 46.5% (n=67) by the IgG-ELISA technique, the difference was significant (p <0.001). We had 2 cases (0.01%) positives by the IgM-ELISA which were not detected by the Toxogen® rapid test.

Conclusion: The Toxogen® rapid test cannot substitute the ELISA technique for the serological screening and/or serological monitoring of toxoplasmosis in pregnant women.

Keywords

ELISA; pregnant women; latex agglutination test; serology; toxoplasmosis

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Copyright (c) 2020 Francine Isabelle Rakotonindrina, Rakotomalala Rivo S, Tsatoromila Fenosoa A, Rabenandrianina Tahirimalala, Rajaonatahina Davidra, Rakotovao Luc A, Rakoto Alson Olivat A, Rasamindrakotroka Andry, Randriamanantany Zely, Razanakolona Lala R S

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