Comparison of the Toxogen® rapid test with ELISA immunoenzymatic technique for toxoplasmosis serology in pregnant women

Francine Isabelle Rakotonindrina(1*), Rakotomalala Rivo S(2), Tsatoromila Fenosoa A(3), Rabenandrianina Tahirimalala(4), Rajaonatahina Davidra(5), Rakotovao Luc A(6), Rakoto Alson Olivat A(7), Rasamindrakotroka Andry(8), Randriamanantany Zely(9), Razanakolona Lala R S(10)
(*) Corresponding Author


The aim of this study was to compare the technical performance of the Toxogen® rapid test against the ELISA technique for serological screening and/or serological monitoring of toxoplasmosis in pregnant women.

Methods and material: A prospective, descriptive and analytical study was conducted over a period of 7 months from January to July 2018. One hundred and eighty-two toxoplasmosis serologies were carried out on 144 pregnant women followed in two hospitals in Mahajanga and in a private laboratory in Antananarivo. Two serologies were performed for each case, the first by the rapid test Toxogen®, a latex agglutination test, and the second by the IgG-ELISA and IgM-ELISA technique.

Results: The Toxogen® rapid test had a sensitivity of 62.5%, a specificity of 97.1%, a Positive Predictive Value of 94.3%, a Negative Predictive Value of 76.7% compared to the IgG-ELISA technique. The correlation between the dilution titer by the Toxogen® rapid test and the optical density (OD) of anti-toxoplasmics IgG by the ELISA technique was very low (r² = 0.17, p <0.001). The crude prevalence of toxoplasmosis was 30,6% (n=44) by the Toxogen® rapid test and 46.5% (n=67) by the IgG-ELISA technique, the difference was significant (p <0.001). We had 2 cases (0.01%) positives by the IgM-ELISA which were not detected by the Toxogen® rapid test.

Conclusion: The Toxogen® rapid test cannot substitute the ELISA technique for the serological screening and/or serological monitoring of toxoplasmosis in pregnant women.


ELISA; pregnant women; latex agglutination test; serology; toxoplasmosis

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Torgerson P, Mastroiacovo P. The global burden of congenital toxoplasmosis : a systematic review. Bull World Health Organ. 2013;91(7):501–8.

Pfister P, Dromigny J. Avidity of IgG anti-Toxoplasma gondii. Study to establish a new decision tree in screening for the disease. Arch Inst Pasteur Madagascar. 2001 ; 67 (1-2) : 57-60.

Yeraa H, Parisc L, Bastiene P, Candolfi E. Biological diagnosis of congenital toxoplasmosis. Revue Francophone des Laboratoires. 2015 ; 470 : 65-72.

Wallon M, Peyron F. Toxoplasmosis. Encycl Med Chir Biologie medicale. 2014;9(4) :90-40-0190-A.

Nizard J. Toxoplasmosis and pregnancy. Journal de Gynécologie Obstétrique et Biologie de la Reproduction. 2008 (37) ; Hors-série 1 :F4-F9.

Mazumder P, Chuang H, Wentz M, Wiedbrauk D. Latex agglutination test for detection of antibodies to Toxoplasma gondii. J Clin Microbiol. 1988 ; 26(11): 2444–6.

Kim Y, Lee J, Ahn S, Kim T-S, Hong S-J et al. Seroprevalence of Toxoplasmosis with ELISA and Rapid Diagnostic Test among Residents in Gyodong-do, Inchon city, Korea: A Four-Year Follow-up. Korean J Parasitol. 2017 ; 55(3) : 247-54.

Saadatnia G, Golkar M. A review on human toxoplasmosis. Scand J Infect Dis. 2012 ; 44(11) : 805-14.

Naghili B, Abbasalizadeh S, Tabrizi S, Rajaii M, Akramiyan M et al. Comparison of IIF, ELISA and IgG avidity tests for the detection of anti-Toxoplasma antibodies in single serum sample from pregnant women. Infez Med. 2017;25(1):50-6.

Drogmigny J, Pecarrere J, Leroy F, Ollivier G, Boisier P. Prevalence of toxoplasmosis in Tananarive. Study conducted at the Pasteur Institute of Madagascar (PIM) on a sample of 2354 subjects. Bull Soc Path Exot. 1996; 89(3) : 212-6.

Khalil MK, Elrayah IE. Seroprevalence of Toxoplasma gondii antibodies in farm animals (camels, cattle, and sheep) in Sudan. Journal of Medicine and Animal Health. 2011 ; 3(3) : 36-9.

Flori P, Chene G, Varlet M, Tran Manh Sung R. Serology of toxoplasmosis in pregnant women: characteristics and pitfalls. Ann Biol Clin. 2009 ; 67(2) : 125-33.

Payne Ra, Francis JM, Kwantes W. Comparison of a latex agglutination test with other serological tests for the measurement of antibodies to Toxoplasma gondii. J Clin Pathol. 1984;37:1293-7.

Villard O, Jung-Etienne J, Cimon B, Franck J, Fricker-Hidalgo H et al. Toxoplasmosis serodiagnosis in 2010: procedure to be followed and interpretation according to the serological profiles obtained by screening methods. Feuillets de Biologie. 2011 ; 52(298) :43-9.

Hounto A, Chokki F, De Tove Y, Bankole B, Adinsi De Souza V et al. Evaluation of a rapid diagnostic test in the diagnosis of toxoplasmosis in pregnant women in Cotonou (Bénin). Bull Soc Pathol Exot. 2014 ; 107(2) : 85-9.

Kosack C, Page A-L, Klatser P. A guide to aid the selection of diagnostic tests. Bull World Health Organ. 2017;95:639-45.

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