Comparison of the Toxogen® rapid test with ELISA immunoenzymatic technique for toxoplasmosis serology in pregnant women

Authors

  • Francine Isabelle Rakotonindrina Laboratory of the Professor Zafisaona Gabriel (PZaGa) University Hospital Center- Faculty of Medicine, University of Mahajanga, Madagascar. https://orcid.org/0000-0002-7608-8219
  • Rakotomalala Rivo S Laboratory of the Professor Zafisaona Gabriel (PZaGa) University Hospital Center- Faculty of Medicine, University of Mahajanga, Madagascar.
  • Tsatoromila Fenosoa A Directorate of Blood Transfusion - Faculty of Medicine, University of Antananarivo.
  • Rabenandrianina Tahirimalala Laboratory of the Professor Zafisaona Gabriel (PZaGa) University Hospital Center- Faculty of Medicine, University of Mahajanga, Madagascar.
  • Rajaonatahina Davidra Paraclinical Training and Research Unit in Immunology - Faculty of Medicine, University of Antananarivo.
  • Rakotovao Luc A CHU / JRB Laboratory - Faculty of Medicine, University of Antananarivo.
  • Rakoto Alson Olivat A CHU / JRB Laboratory - Faculty of Medicine, University of Antananarivo.
  • Rasamindrakotroka Andry Laboratory for Training and Research in Medical Biology, Faculty of Medicine, University of Antananarivo.
  • Randriamanantany Zely Directorate of Blood Transfusion - Faculty of Medicine, University of Antananarivo.
  • Razanakolona Lala R S National Reference Laboratory for HIV / AIDS and Sexually Transmitted Infections - Ministry of Public Health - Faculty of Medicine, University of Antananarivo.

DOI:

https://doi.org/10.5281/zenodo.3931160

Keywords:

ELISA, pregnant women, latex agglutination test, serology, toxoplasmosis

Abstract

The aim of this study was to compare the technical performance of the Toxogen® rapid test against the ELISA technique for serological screening and/or serological monitoring of toxoplasmosis in pregnant women.

Methods and material: A prospective, descriptive and analytical study was conducted over a period of 7 months from January to July 2018. One hundred and eighty-two toxoplasmosis serologies were carried out on 144 pregnant women followed in two hospitals in Mahajanga and in a private laboratory in Antananarivo. Two serologies were performed for each case, the first by the rapid test Toxogen®, a latex agglutination test, and the second by the IgG-ELISA and IgM-ELISA technique.

Results: The Toxogen® rapid test had a sensitivity of 62.5%, a specificity of 97.1%, a Positive Predictive Value of 94.3%, a Negative Predictive Value of 76.7% compared to the IgG-ELISA technique. The correlation between the dilution titer by the Toxogen® rapid test and the optical density (OD) of anti-toxoplasmics IgG by the ELISA technique was very low (r² = 0.17, p <0.001). The crude prevalence of toxoplasmosis was 30,6% (n=44) by the Toxogen® rapid test and 46.5% (n=67) by the IgG-ELISA technique, the difference was significant (p <0.001). We had 2 cases (0.01%) positives by the IgM-ELISA which were not detected by the Toxogen® rapid test.

Conclusion: The Toxogen® rapid test cannot substitute the ELISA technique for the serological screening and/or serological monitoring of toxoplasmosis in pregnant women.

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Author Biography

Francine Isabelle Rakotonindrina, Laboratory of the Professor Zafisaona Gabriel (PZaGa) University Hospital Center- Faculty of Medicine, University of Mahajanga, Madagascar.

Medical biologist

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